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In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 54, Heft 1, S. 72-79
ISSN: 2524-2342
In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 54, Heft 2, S. 197-203
ISSN: 2524-2342
In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Band 56, Heft 2, S. 197-205
ISSN: 2524-2342
We report herein improved version of the synthesis of hapten based on cholecalciferol and its active metabolite 25-hydroxycholecalcalferol. The methodology of obtaining high-molecular immunogenic conjugates of vitamin D3 derivatives with bovine serum albumin and horseradish peroxidase conjugates for direct ELISA was optimized. Immunisation of rabbits was carried out and polyclonal antibodies to 25-hydroxycholecalceferol were obtained and tested in an enzyme-linked immunosorbent assay. To improve the accuracy of the method, the sample preparation procedure was optimized, including the release of vitamin D3 and its active metabolites from complexes with vitamin D-binding protein.